60050 Coliform Test Paper

Estimated delivery : 30 Days; MOQ : 10 Box (1 Box = 10 PCS)
Model Number : 60050
Test Range : 0.05-0.1-0.2-0.3-0.4-0.5-0.7-1mg/L

Product Description :
Application: Widely used in drinking water, natural mineral water for drinking coliform detection of source water.

Inspection methods
1.Inoculation
According to contamination of samples, not diluted or select the appropriate dilution, each draw five of 10 mL and applied to the five large paper bag; five of 1 mL applied to five bags of small pieces of paper; then another 1 mL aliquot diluted 10-fold, draw five 1 mL were applied to five bags of small pieces of paper. Then test the paper by hand gently flatten the bag vertically upward to 18 ~ 24h culture incubator (35 ~ 37 ℃) inside, and then observe the results.
2.Results to determine
Negative results: violet blue paper unchanged, regardless of flush or red spots which is judged as coliform negative.
Positive results: showing red spots or flush on the paper, and its surrounding yellow or yellow throughout the paper which is judged as positive for coliform bacteria.
3.Result report
Coliform MPN check the appropriate table based on the number of positive reaction paper reports per 100mL MPN values in the detection of coliform bacteria samples (most probable number).

Note
1.Should be protected from light hypothermia (10 ℃ below) to save, and pay attention to moisture, if pink was found in the paper then the product is unavailable.
2.Confirmatory test methods
To make confirmation test, using sterile forceps aseptically suspicious paper transplanted to lactose fermentation tube, set in 37℃ culture for 24 hours, its produce acid and gas were judged as positive for coliform bacteria, otherwise judged as negative .

Test principle
The nutrients adsorbed on the filter paper are used as the culture matrix for coliform bacteria. Coliform bacteria can ferment lactose in the culture medium to produce acid, causing the bromine violet indicator to change from purple to yellow. The TTC in the culture medium is reduced by bacteria to form easily identifiable red colonies. The packaging is aseptically processed and can be directly used for the detection of coliform bacteria in food, beverages and drinking pure water.